Composite
NG9-2

Part:BBa_K2623031

Designed by: Junhong Chen   Group: iGEM18_XMU-China   (2018-10-07)


A composite part linked by BBa_K2623024 and BBa_K2623026

Under the induction of IPTG, this part can code OmpA-SpyTag and SpyCatcher-L7Ae to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatcher. It's a non-RFP form of BBa_K2623030 and another form of BBa_K2623029.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1965
    Illegal NheI site found at 1988
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None